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recombinant murine tweak  (R&D Systems)


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    R&D Systems recombinant murine tweak
    Recombinant Murine Tweak, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+mouse+tweak/Recombinant+Mouse+TWEAK%2FTNFSF12+Protein/pm41927716-229-10-14
    Average 94 stars, based on 9 article reviews
    recombinant murine tweak - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Recombinant:

    Article Title: TWEAK/Fn14 system and crescent formation in IgA nephropathy
    Article Snippet: .. Recombinant mouse TWEAK (R&D Systems) at 0–1000 ng/ml was added at the beginning of the experiment. .. After stimulation of MMC with TWEAK for 24 hours, cell proliferation ELISA using BrdU was performed with a colorimetric immunoassay kit (Roche Diagnostics, Mannheim, Germany) following the manufacturer’s instructions.

    Article Title: TNF-Related Weak inducer of Apoptosis (TWEAK) is a potent skeletal muscle-wasting cytokine
    Article Snippet: Protein A sepharose beads were obtained from Pierce Biotechnology (Rockford, IL). .. Recombinant mouse TWEAK, TNF-α protein and TWEAK ELISA kit were from R&D Systems (Minneapolis, MN). .. Annexin V-EGFP apoptosis detection kit was obtained from BioVision Inc. (Mountain View, CA).

    Article Title: The deposition of anti-DNA IgG contributes to the development of cutaneous lupus erythematosus.
    Article Snippet: Anti-DNA IgG is a hallmark serum of systemic lupus erythematosus and induces internal injuries in patients.. It is known that cutaneous lupus erythematosus (CLE) involves the deposition of autoantibodies in the dermoepidermal junction of the skin and that anti-DNA IgG binds specifically to keratinocytes.. However, the definite role of anti-DNA IgG in CLE remains unclear.

    Article Title: TWEAK/Fn14 Activation Participates in Ro52-Mediated Photosensitization in Cutaneous Lupus Erythematosus.
    Article Snippet: .. In some experiments, culture media were added with recombinant mouse TWEAK (100 ng/ml, 2 days; R&D Systems, Minneapolis, MN, USA) or bovine serum albumin (BSA; 100 ng/ml, 2 days; Sigma-Aldrich, St. Louis, MO, USA) immediately after UVB irradiation. .. The inhibitors of JSH-23 (20 μM; Sigma-Aldrich), U0126 (10 μM; Cell signaling, Danvers, MA, USA), and wortmannin (25 μg/ml; Beyotime Biotech) were added 2 h before the UVB and TWEAK treatments.

    Article Title: TWEAK/Fn14 Activation Participates in Ro52-Mediated Photosensitization in Cutaneous Lupus Erythematosus
    Article Snippet: .. In some experiments, culture media were added with recombinant mouse TWEAK (100 ng/ml, 2 days; R&D Systems, Minneapolis, MN, USA) or bovine serum albumin (BSA; 100 ng/ml, 2 days; Sigma-Aldrich, St. Louis, MO, USA) immediately after UVB irradiation. .. The inhibitors of JSH-23 (20 μM; Sigma-Aldrich), U0126 (10 μM; Cell signaling, Danvers, MA, USA), and wortmannin (25 μg/ml; Beyotime Biotech) were added 2 h before the UVB and TWEAK treatments.

    Article Title: NF-κB inducing kinase is a therapeutic target for systemic lupus erythematosus
    Article Snippet: Murine RPTEC (Sciencell) were cultured as suggested by the vendor. .. 5 × 10 6 cells were stimulated with recombinant mouse TWEAK (100 ng/mL, R&D Systems) for 6 and 24 h in the presence or absence of 3 μM NIK SMI1 (~ mouse IC 90 ) or vehicle (DMSO) in 3 replicates per treatment. .. Equivalent stimulations were also done with human RPTEC cells (LONZA) stimulated with rhTWEAK and 1 μM NIK SMI1 (~human IC 90 ) for 6 and 24 h for RT-PCR measurements.

    Article Title: TWEAK /Fn14 mediates atrial‐derived HL ‐1 myocytes hypertrophy via JAK 2/ STAT 3 signalling pathway
    Article Snippet: .. Recombinant mouse TWEAK was purchased from R&D Systems (Minneapolis, MN, USA) and reconstituted with sterile phosphate‐buffered saline (PBS) to 100 μg/mL and stored at −20°C. ..

    Article Title: Inhibition of suppressor of cytokine signaling 1 mediates the profibrotic effect of TWEAK/Fn14 signaling on kidney cells.
    Article Snippet: Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) engagement with the receptor Fn14 contributes to the fibrotic process of kidney cells in systemic lupus erythematosus.. Downregulation of the protein suppressor of cytokine signaling 1 (SOCS1) correlates with amplified production of proinflammatory factors and cell apoptosis, which participate in the pathogenesis of lupus nephritis.. To elucidate the potential role of SOCS1 in TWEAK/Fn14 signaling, we determined the SOCS1 levels in primary kidney cells from MRL/MpJ (control strain) or MRL/lpr (lupus-prone) mice.

    Enzyme-linked Immunosorbent Assay:

    Article Title: TNF-Related Weak inducer of Apoptosis (TWEAK) is a potent skeletal muscle-wasting cytokine
    Article Snippet: Protein A sepharose beads were obtained from Pierce Biotechnology (Rockford, IL). .. Recombinant mouse TWEAK, TNF-α protein and TWEAK ELISA kit were from R&D Systems (Minneapolis, MN). .. Annexin V-EGFP apoptosis detection kit was obtained from BioVision Inc. (Mountain View, CA).

    Control:

    Article Title: The deposition of anti-DNA IgG contributes to the development of cutaneous lupus erythematosus.
    Article Snippet: Anti-DNA IgG is a hallmark serum of systemic lupus erythematosus and induces internal injuries in patients.. It is known that cutaneous lupus erythematosus (CLE) involves the deposition of autoantibodies in the dermoepidermal junction of the skin and that anti-DNA IgG binds specifically to keratinocytes.. However, the definite role of anti-DNA IgG in CLE remains unclear.

    Irradiation:

    Article Title: TWEAK/Fn14 Activation Participates in Ro52-Mediated Photosensitization in Cutaneous Lupus Erythematosus.
    Article Snippet: .. In some experiments, culture media were added with recombinant mouse TWEAK (100 ng/ml, 2 days; R&D Systems, Minneapolis, MN, USA) or bovine serum albumin (BSA; 100 ng/ml, 2 days; Sigma-Aldrich, St. Louis, MO, USA) immediately after UVB irradiation. .. The inhibitors of JSH-23 (20 μM; Sigma-Aldrich), U0126 (10 μM; Cell signaling, Danvers, MA, USA), and wortmannin (25 μg/ml; Beyotime Biotech) were added 2 h before the UVB and TWEAK treatments.

    Article Title: TWEAK/Fn14 Activation Participates in Ro52-Mediated Photosensitization in Cutaneous Lupus Erythematosus
    Article Snippet: .. In some experiments, culture media were added with recombinant mouse TWEAK (100 ng/ml, 2 days; R&D Systems, Minneapolis, MN, USA) or bovine serum albumin (BSA; 100 ng/ml, 2 days; Sigma-Aldrich, St. Louis, MO, USA) immediately after UVB irradiation. .. The inhibitors of JSH-23 (20 μM; Sigma-Aldrich), U0126 (10 μM; Cell signaling, Danvers, MA, USA), and wortmannin (25 μg/ml; Beyotime Biotech) were added 2 h before the UVB and TWEAK treatments.

    Sterility:

    Article Title: TWEAK /Fn14 mediates atrial‐derived HL ‐1 myocytes hypertrophy via JAK 2/ STAT 3 signalling pathway
    Article Snippet: .. Recombinant mouse TWEAK was purchased from R&D Systems (Minneapolis, MN, USA) and reconstituted with sterile phosphate‐buffered saline (PBS) to 100 μg/mL and stored at −20°C. ..

    Saline:

    Article Title: TWEAK /Fn14 mediates atrial‐derived HL ‐1 myocytes hypertrophy via JAK 2/ STAT 3 signalling pathway
    Article Snippet: .. Recombinant mouse TWEAK was purchased from R&D Systems (Minneapolis, MN, USA) and reconstituted with sterile phosphate‐buffered saline (PBS) to 100 μg/mL and stored at −20°C. ..

    Blocking Assay:

    Article Title: Inhibition of suppressor of cytokine signaling 1 mediates the profibrotic effect of TWEAK/Fn14 signaling on kidney cells.
    Article Snippet: Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) engagement with the receptor Fn14 contributes to the fibrotic process of kidney cells in systemic lupus erythematosus.. Downregulation of the protein suppressor of cytokine signaling 1 (SOCS1) correlates with amplified production of proinflammatory factors and cell apoptosis, which participate in the pathogenesis of lupus nephritis.. To elucidate the potential role of SOCS1 in TWEAK/Fn14 signaling, we determined the SOCS1 levels in primary kidney cells from MRL/MpJ (control strain) or MRL/lpr (lupus-prone) mice.



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    Fig. 4. <t>FN14</t> decoy receptor modulates the cytokine profile in CNV and reduces neovascularization size. (A): Experimental setup. (B): Intravitreal injection of a FN14 decoy receptor compared to PBS (control) significantly reduced CNV size. For statistical analysis, Mann–Whitney U test was applied. Color fundus (CF) and fluo rescence angiography (FA) on d7 of representative eyes of the FN14 decoy receptor (upper panel) and the PBS group (lower panel) as well as confocal microscopy (CM) images of representative lesions (COL4 in red) the size of which approximately corresponds to the mean spot size within the respective group. (C): Protein concentrations of several cytokines on d5 in the RPE of unlasered controls, CNV injected with PBS (CNV) or with FN14 decoy receptor on d1 (CNV + decoy). Data are shown as mean with SEM. For statistical analysis, paired ANOVA adjusting for total protein concentration was applied. *: p < 0.05, ns: not significant.
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    Image Search Results


    Fig. 4. FN14 decoy receptor modulates the cytokine profile in CNV and reduces neovascularization size. (A): Experimental setup. (B): Intravitreal injection of a FN14 decoy receptor compared to PBS (control) significantly reduced CNV size. For statistical analysis, Mann–Whitney U test was applied. Color fundus (CF) and fluo rescence angiography (FA) on d7 of representative eyes of the FN14 decoy receptor (upper panel) and the PBS group (lower panel) as well as confocal microscopy (CM) images of representative lesions (COL4 in red) the size of which approximately corresponds to the mean spot size within the respective group. (C): Protein concentrations of several cytokines on d5 in the RPE of unlasered controls, CNV injected with PBS (CNV) or with FN14 decoy receptor on d1 (CNV + decoy). Data are shown as mean with SEM. For statistical analysis, paired ANOVA adjusting for total protein concentration was applied. *: p < 0.05, ns: not significant.

    Journal: Biochimica et biophysica acta. Molecular basis of disease

    Article Title: Comparative transcriptome analysis of human and murine choroidal neovascularization identifies fibroblast growth factor inducible-14 as phylogenetically conserved mediator of neovascular age-related macular degeneration.

    doi: 10.1016/j.bbadis.2022.166340

    Figure Lengend Snippet: Fig. 4. FN14 decoy receptor modulates the cytokine profile in CNV and reduces neovascularization size. (A): Experimental setup. (B): Intravitreal injection of a FN14 decoy receptor compared to PBS (control) significantly reduced CNV size. For statistical analysis, Mann–Whitney U test was applied. Color fundus (CF) and fluo rescence angiography (FA) on d7 of representative eyes of the FN14 decoy receptor (upper panel) and the PBS group (lower panel) as well as confocal microscopy (CM) images of representative lesions (COL4 in red) the size of which approximately corresponds to the mean spot size within the respective group. (C): Protein concentrations of several cytokines on d5 in the RPE of unlasered controls, CNV injected with PBS (CNV) or with FN14 decoy receptor on d1 (CNV + decoy). Data are shown as mean with SEM. For statistical analysis, paired ANOVA adjusting for total protein concentration was applied. *: p < 0.05, ns: not significant.

    Article Snippet: At d1 mice received an intravitreal injection of 4 μg FN14 decoy receptor (1610- TW, R&D Systems) dissolved in 1 μl PBS in one eye and the same volume of PBS in the other eye.

    Techniques: Injection, Control, MANN-WHITNEY, Confocal Microscopy, Protein Concentration